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. 2018 May 2;8:6889. doi: 10.1038/s41598-018-25236-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of MAYV directly from mosquito saliva through RT-PCR at 28 dpi. It was only possible to detect virus in Br mosquitoes (fresh and frozen). However, the numbers virus copies were lower when using frozen virus. It was not possible to detect virus in wMel mosquito saliva. The Fisher’s exact test showed no significant differences between fresh and frozen virus for Br mosquitoes (P = 0.1698).