Fig. 2.

Quorum-sensing induction of cdeA and cdeB transcription. Quantitative digital PCR was used to quantify cdeA and cdeB transcripts in cells from stationary-phase cultures (optical density of 600 nm [OD₆₀₀] of 4). Shown are cdeA and cdeB transcripts from wild type (WT, black bars), the AHL synthase, receptor double mutant (CviIR⁻, gray bars), and the bactobolin-resistant double mutant (CviIR⁻ BR, white bars). In all cases, results were normalized to the housekeeping gene encoding glyceraldehyde-3-phosphatase dehydrogenase (gapdh). The values represent the average of three independent experiments and the error bars represent the standard deviation. Statistical analysis by t-test compared with wild type: *, p ≤ 0.005; **, p ≤ 0.05