Fig. 4.

Vγ9⁻ Vδ2⁺ T cells undergo clonal expansion. a Identification of Vγ9⁻ Vδ2⁺ T cells (left) and CD27/CD45RA T-cell memory marker expression on Vγ9⁻ Vδ2⁺ T cells (right) in a peripheral blood sample from “donor X”. b Single-cell CDR3δ sequence analysis and Vγ usage by Vγ9⁻ Vδ2⁺ T cells sorted from a peripheral blood sample from “donor X”. c TCRγ repertoire analysis of Vδ2⁺ T cells from “donor X”, highlighting the frequency of “donor X’s” Vγ9⁻ Vδ2⁺ T-cell clone (from b) within the top 10 most prevalent clonotypes, highlighted are prevalent Vγ9⁺ clonotypes (EVQ, EAQ, EVR and EQE). d Analysis of naive and effector T-cell marker expression by Vγ9⁻ Vδ2⁺, CD8⁺ CD27^hi CD45RA^hi (CD8⁺ T_naive) and CD27^lo/neg Vδ1⁺ T-cell populations from a peripheral blood sample from “donor X”