Fig. 5.

Clonally expanded Vγ9⁻ Vδ2⁺ T cells infiltrate human liver tissue. a Representative identification of intrahepatic Vγ9⁻ Vδ2⁺ T cells in CD3⁺ lymphocytes obtained from liver tissue and b summary data of the frequency of Vδ2⁺ T cells in CD3⁺ T cells (left) or Vγ9⁻ Vδ2⁺ T cells in Vδ2⁺ T cells (right) from peripheral blood (n = 18) and liver tissue (n = 16). c Representative intrahepatic Vγ9⁻ Vδ2⁺ T-cell expression profiles of CD27 and CD45RA T-cell memory markers and d summary data from peripheral blood (n = 18) and liver tissue (n = 16). e Single-cell CDR3δ sequence analysis and Vγ usage by intrahepatic Vγ9⁻ Vδ2⁺ T cells sorted from two independent liver tissue samples. f Representative analysis of CX₃CR1, IL7Rα and Granzyme A expression by intrahepatic Vγ9⁻ Vδ2⁺ T cells (n = 3) and a healthy donor’s (HD) CD27^lo/neg Vδ1⁺ T-cell population (n = 8). Error bars indicate means ± SEM; ****P < 0.0001; p-values were determined by Mann–Whitney t-test (b, d)