Table 2.
Single-cell TCR sequencing of Vγ9− Vδ2+ T cells
| Donor | CDR3δ2 | Dδ | Jδ | CDR3δ length | Vγ | Jγ | CDR3γ length |
|---|---|---|---|---|---|---|---|
| 3 | CACGSWWGTYTDKLIF | 1,3 | 1 | 14 | 8 | P1 | 11 |
| 231 | CACDTIVGDTLTDKLIF | 3 | 1 | 15 | 8 | P1 | 9 |
| CACVRGGGYPRGADKLIF | 3 | 1 | 16 | 8 | 1/2 | 14 | |
| CACDTEGEVNTDKLIF | 3 | 1 | 14 | 4 | P1 | 11 | |
| 261 | CACDTRFRPGRSARVVRLTAQLFF | 1 | 2 | 22 | 3 | P1 | 14 |
| Liver 1 | CACSREQAHTDKLIF | 3 | 1 | 13 | 3 | 1/2 | 10 |
| CACDTGWGIRGRYTDKLIF | 3 | 1 | 17 | 4 | 1/2 | 11 | |
| Liver 2 | CACDRSGARWDKLIF | — | 1 | 13 | 5 | 1/2 | 10 |
| 42 | CACDARHYWGISTDKLIF | 3 | 1 | 16 | 2 | 1/2 | 12 |
| CACDARLGEHTDKLIF | 3 | 1 | 14 | ND | ND | ND | |
| CACDRMGDLPSSWDTRQMFF | 3 | 3 | 18 | 3 | 1/2 | 13 | |
| CACGSSWGVSHYTDKLIF | 3 | 1 | 16 | 5 | 1/2 | 10 | |
| CACDLLGDFRTDKLIF | 3 | 1 | 14 | 8 | 1/2 | 8 | |
| CACSVRGHWGRSTDKLIF | 3 | 1 | 16 | 8 | 1/2 | 14 | |
| CACDTRGIGDTLPDKLIF | 3 | 1 | 16 | 8 | P1 | 12 | |
| CACAHGYWGTPWDTDKLIF | 3 | 1 | 17 | 3 | P1 | 11 | |
| CACDTGDTDTDKLIF | 3 | 1 | 13 | 8 | P1 | 13 | |
| CACDRLLLGDTDKLIF | 3 | 1 | 14 | ND | ND | ND | |
| CACDTRRTGGRDKLIF | 3 | 1 | 14 | 5 | 1/2 | 9 | |
| CACDKRIRWGNPYTDKLIF | 3 | 1 | 17 | 8 | P2 | 10 | |
| CACEVPSYEPYWGTKKYTDKLIF | 2,3 | 1 | 21 | 3 | 1/2 | 11 | |
| CACDTGDWGINTDKLIF | 3 | 1 | 15 | 8 | P1 | 14 | |
| CACDQKYWGGSSTDKLIF | 3 | 1 | 16 | 2 | P | 13 |
TCRδ sequences of clonally expanded Vγ9− Vδ2+ TCRs from donors expressing a CD27lo/neg phenotype (top section). All TCRδ2 sequences from a representative healthy donor with a CD27hi phenotype (bottom section). Sequences were analysed using IMGT Junction Analysis, which identified V, D and J gene segments used. The table depicts CDR3δ sequences, Dδ usage, Jδ usage, CDR3 length, Vγ usage, Jγ usage and CDR3γ length. Amino acids (aa) at position 5 of the CDR3 sequence (see Methods) are highlighted in bold. ND not determined