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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Mol Cancer Ther. 2018 Feb 26;17(5):1070–1078. doi: 10.1158/1535-7163.MCT-17-1053

Figure 3.

Figure 3

Influence of GSKJ4 on H3K27me3 levels in and radiosensitivity of the 3 tumor cell lines. A) GSKJ4 (4μM) was added to culture media immediately before irradiation (10Gy), 1h later chromatin protein was collected and subjected to immunoblot analysis for H3K27me3 with H3 used as a loading control. Representative immunoblots from each cell line are presented with the ratios comparing H3K27me3 levels in treated versus untreated cells shown below each blot. B) For clonogenic survival analysis GSKJ4 (4μM) was added to culture media immediately prior to irradiation, 24h later cultures were rinsed and fed fresh, drug-free medium. Colony-forming efficiency was determined 10 to 14 days later and survival curves were generated after normalizing for cell killing from drug alone. DEFs were calculated at a surviving fraction of 0.1. Values shown represent the mean ± SEM for 3 to 4 independent experiments. *p≤0.04 according to Student’s t test.