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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Cell Metab. 2018 Apr 12;27(5):1121–1137.e5. doi: 10.1016/j.cmet.2018.03.005

Figure 3. Temperature-sensitive plasticity of beige adipocyte chromatin.

Figure 3

(a) H3K27ac ChIP-seq data centered on the TSS (±5kb) of general adipocyte, thermogenic, and white adipocyte marker genes.

(b) PCA of H3K27ac peaks from different adipocyte types in cold and warm conditions. Each dot indicates an individual biological replicate (a pool of 3–6 animals) analyzed in two independent experiments.

(c) Heatmap of pairwise comparisons for the number of differentially-regulated H3K27ac peaks. Values indicate the percentage and number (in parentheses) of differentially-regulated peaks in each comparison. Total number of H3K27ac peaks is 37,254.

(d) Scatter plot showing correlation of the changes of all individual H3K27ac peak signals upon warming between brown and beige adipocytes. The red dashed line is the trend line.

(e) Heatmap showing H3K27ac peaks (rows) across samples (columns). Peaks are classified into 6 clusters based on their patterns as labeled. Amplitude of each peak center (±5kb) is represented in color as indicated.

(f) Pie chart showing the number of H3K27ac peaks in each cluster. The number of H3K27ac peaks in each cluster is indicated.

See also Figures S3 and S4.