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. 2018 Apr 26;9:817. doi: 10.3389/fmicb.2018.00817

FIGURE 4.

FIGURE 4

EV71-induced cytopathic effects are blocked by caspase-3 inhibitor. RD cells were treated with 20 μM of caspase-3 inhibitor (In) or 0.05% DMSO in 10% DMEM (Con) for 2 h, and then were mock-infected (Mock) or infected with EV71 (EV) at an MOI of 1. After 2 h, the cells were re-treated with caspase-3 inhibitor (In) or 0.05% DMSO in 10% DMEM (Con) for another 22 h. (A) Morphologic analysis of the effect of caspase-3 inhibitor on cell death after EV71 infection. Cell morphology was visualized by light microscopy. Arrows indicate dead cells. Bar = 20 μm. The results are representative of three independent experiments. (B) Nuclear morphologic analysis of the effect of caspase-3 inhibitor on cell death after EV71 infection. The nuclear morphology was visualized by light microscopy after Hoechst 33258 staining. Arrows indicate dead cells. Bar = 10 μm. The results are representative of three independent experiments. (C) Cell number analysis of the effect of caspase-3 inhibitor on cell death after EV71 infection. The cell numbers were counted after trypan blue staining. (D) Effect of caspase-3 inhibitor on the release of inflammatory cytokine. The level of IL-6 in cultural medium was assessed by ELISA. The results show the means ± SD of three independent experiments. ∗∗∗P < 0.001.