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. 2018 Apr 26;9:266. doi: 10.3389/fneur.2018.00266

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Copyright © 2018 Ayana, Singh and Pati.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of hESC-derived microglia precursors in vitro using different stage-specific markers. (A) Flow cytometry based analysis demonstrated characterization of microglia induction and differentiation of microglial precursors. The analysis indicated differentiation of hESCs into embryonic bodies (EB, 5 DIV), as shown by expression of ~49.7% CD24^Pos and 11.4% CD184^Pos population. This is followed by neural induction as shown by 20% of NESTIN^Pos cells. Neurally induced cells further showed the generation of a distinct population having 54% of CD68^Pos cells and 91% IBA1^Pos cells. (B) Confocal imaging of hESC-derived EBs showed expression of CD24 and CD184 in vitro at 5 DIV. The emergence of mature microglial precursors could be detected on 34DIV, as shown by IBA1, SPHK1, and PTX3 expression. DAPI = nuclear marker, DIV = days in vitro. The scale bar represents size in micrometers.