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. 2018 Apr 26;11:141. doi: 10.3389/fnmol.2018.00141

Figure 3.

Figure 3

PKC inhibition prevents a single EtOH withdrawal-induced reduction in cell-surface level of native GABAAR-δ subunits and total protein level of recombinant δ subunits. (A) Representative western blots (top panels) and corresponding quantification (lower panel) of the biotinylation assay for cell-surface GABAAR-δ level in neurons from Ctrl, E/W, Che+E/W, and Che groups. β-actin of total cell lysate was used as the loading control. n = 4–5 per group. (B) Representative time-lapse live-cell images of pcDNA3.1-GABAAR-δ-mCherry transfected-neurons from EtOH and Che+EtOH treatments (top panels) and corresponding quantification of changes in recombinant δ subunit intensity in neurons from vehicle, EtOH, Che+EtOH, and Che treatments, respectively (lower panel). (C) Representative time-lapse live-cell images of pcDNA3.1-GABAAR-α1-EGFP transfected-neurons from EtOH and Che+EtOH treatments (top panels) and corresponding quantification of recombinant α1 subunit intensity in neurons from vehicle, EtOH, Che+EtOH, and Che treatments, respectively (lower panel). Scale bars = 10 μm. n = 19–21 per group. *p < 0.05, **p < 0.01 (one-way ANOVA with post hoc Dunnett’s test); †p < 0.05 vs. the pre-drug baseline value, #p < 0.05 vs. vehicle group (two-way ANOVA with post hoc Holm-Sidak test).