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. 2018 Apr 26;9:557. doi: 10.3389/fpls.2018.00557

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Copyright © 2018 Shi, Melzer, Zheng, Benke, Stich and von Wirén.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of varied Fe and P nutritional regimes on ferritin gene expression. Relative transcript levels of ZmFer1 (A,B) and ZmFer2 (C,D) in shoots (A,C) and roots (B,D) of B73 and Mo17 plants grown for 15 days in nutrient solution supplemented without Fe and P (Fe0P0), without Fe and with 100 μM P (Fe0P100), with 100 μM Fe and without P (Fe100P0) or with 100 μM Fe and 100 μM P (Fe100P100). Fe and P treatments were initiated after one week of preculture on full nutrient solution. The housekeeping gene ZmGAPDH was used for normalization of mRNA levels, which were set to 1.0 in B73 grown at Fe100P100. Bars indicate mean values ± SD, n = 4. Different letters denote significant differences at p < 0.05.