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Purification of the citrate carrier from maize mitochondria. Results of SDS-gel electrophoresis of fractions obtained by hydroxyapatite and hydroxyapatite/celite columns of maize mitochondria solubilized with Triton X-100 are shown. Lane M, Protein markers (from top to bottom: BSA, carbonic anhydrase, and Cyt c); lane 1, Triton X-100 mitochondrial extract (180 μg in 25 μL); lane 2, hydroxyapatite eluate (20 μg in 100 μL); lane 3, hydroxyapatite/celite eluate (2 μg in 160 μL).
