Table II.
Dependence of citrate transport in reconstituted liposomes on internal substrates
| Internal Substrate (20 mm) | Citrate Transport |
|---|---|
| nmol 10 min−1 mg−1 protein | |
| None (Cl− present) | 420 |
| Citrate | 6180 |
| cis-Aconitate | 4400 |
| Isocitrate | 3640 |
| trans-Aconitate | 710 |
| 1,2,3-Benzenetricarboxylate | 580 |
| Oxalate | 865 |
| Malonate | 4950 |
| l-Malate | 5300 |
| Succinate | 5070 |
| Glutarate | 3065 |
| Adipate | 1850 |
| Pimelate | 550 |
| Oxaloacetate | 6000 |
| α-Ketoglutarate | 6080 |
| α-Ketoadipate | 2080 |
| α-Ketopimelate | 350 |
| PEP | 865 |
| Asp | 515 |
| Asn | 495 |
| Glu | 490 |
| Gln | 520 |
| Lys | 490 |
| Phosphate | 530 |
| Pyruvate | 805 |
| ADP | 915 |
The proteoliposomes were loaded with the indicated substrates. Transport was initiated by adding 0.1 mm [14C]citrate. The results are the means of three experiments.