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. 2018 Apr 26;9:439. doi: 10.3389/fphys.2018.00439

Figure 3.

Figure 3

Functional properties of Hif1a cDNA mutants. Relative levels of Hif-1α target genes mRNA expression in (A) WT MS5 cell line, (B) Hif1a−/− MS5 Clone 41 cell line, (C) Hif1a−/− MS5 Clone 41 cell line transduced with a WT Hif-1a cDNA lentiviral construct, (D) Hif1a−/− MS5 Clone 41 cell line transduced with a bHLH-mutated Hif1a cDNA lentiviral construct, and (E) Hif1a−/− MS5 Clone 41 cell line transduced with a PAS-A-mutated Hif1a cDNA lentiviral construct, and (F) Hif1a−/− MS5 Clone 41 cell line transduced with an empty lentiviral construct, cultured in 21 or 2% O2. Expression levels were calculated according to the ΔΔCt method normalizing against β-Actin (housekeeping gene) and considering 21% O2 as the reference condition for the calculations. *p < 0.05 paired t-tests, n = 3.