Table 2.

Summary of the effects of the environmental toxins on cardiac spheroids.

Environmental toxins on cardiac organoids	Range of Doses	Live/Dead Effective Dose	IC50	Heart beat effective dose at 30 min	Heart beat effective dose at 24 h	Heart beat effective dose at 48 h
Glyphosate	25 µM–25 mM	2.5–25 mM	10.85 mM	2.5 mM	2.5 mM	2.5 mM
Lead	10 µM–10 mM	1–10 mM	2.45 mM	10 mM	100 µM	100 µM
Mercury	200 nM–200 µM	2–20 µM	44 µM	2 µM	2 µM	2 µM
Thallium	10 nM–10 µM	10–100 µM	1.35 µM	10 µM	1 µM	1 µM

Effective dose for live/dead imaging is summarized as a qualitative measure of rapid cell death detected in images. Effective dose for heart beat assay at each time point is described by a p-value of 0.05.