FIGURE 6.

In vitro and in vivo oxidative stress assessments. (A) Oxidative stress marker protein 8-OHdG was used to detect cisplatin-induced damages (positive cells were marked in green with arrowheads). Cisplatin treatment increased detection of 8-OHdG while co-incubation of HNKl significantly reduced cellular oxidative. (B) Quantification analysis showed that cells that were positive for oxidative stress decreased from 44.3 to 20.3% and from 66.4% in cisplatin-treated group to 45% when HNK was co-incubated. Time-dependent anti-oxidant analyses revealed significant reduction of 8-OHdG positive cells in HNK-treated group, and the peak effect appeared at 8 h after the incubation and gradually diminished after 12–24 h. Data were presented as mean ± SEM. Images presented were representative images. Statistical significant difference between groups were marked with ^∗∗p < 0.01, ^∗0.05 < p < 0.1.