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Stimuli and inhibitors used.
| Concentration | Cat # | Supplier | |
|---|---|---|---|
| STIMULI | |||
| PMA | 200 ng/ml | P8139 | Sigma |
| Ionomycin | 1 μM | CALB407952 | Merck millipore |
| Anti-CD3 | 10 μg/ml | 553057 | BD pharmingen |
| Anti-CD28 | 10 μg/ml | 553294 | BD pharmingen |
| TNF | 1,000 U/ml | – | VIB protein service facility |
| INHIBITORS | |||
| MRT67307 | 1–10 μM | HY-13018 | MedChemtronica |
| TPCA1 | 5 μM | 2559 | Tocris biosciences |
