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. 2018 Apr 18;2018:5736535. doi: 10.1155/2018/5736535

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Copyright © 2018 Mi-Young Moon et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Zinc induces neuronal gene expression in differentiated AD-MSCs. Effects of zinc on the expression of NES and MAP2 during neuronal differentiation in AD-MSCs. (a, b) To investigate whether zinc played a role in the expression of the surface protein molecules CD90 and CD105, flow cytometry was performed in differentiated AD-MSCs. AD-MSCs were incubated in differentiation medium with the presence of either 10 μM ZnCl₂ or CaEDTA for 3 days. Analysis of the expression of CD90 and CD105 was determined by WinMDI software. (c, d) mRNA expression of NES and MAP2 was measured by real-time quantitative RT-PCR. These data represent the mean ± SD from three independent experiments (^∗p < 0.05 versus untreated with ZnCl₂; ^#p < 0.05 versus treated with ZnCl₂).