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The time-dependent effects on the behavioral responses of rats exposed to the AIH or PPI tests by tap water, CGMP (1500 mg/kg)+Leu (300 mg/kg), CGMP (1500 mg/kg)+Leu (300 mg/kg)+Trp (45 mg/kg), BCAA mixture (Leu 600 mg/kg+Ile 450 mg/kg+Val 450 mg/kg), or whey protein (1500 mg/kg). Independent groups of rats were exposed to the AIH or PPI after 2 h, 8 h, or 1 week (daily) of oral gavage treatment. Values are expressed as the mean±SEM. 1-way ANOVA followed by Tukey’s posthoc, *P<.05.
