Figure 4.

EBER1 and derivatives do not have direct miRNA-like activity. (A) Schematic representation of the luciferase EBER1 3′UTR reporter construct generated in the vector pGL3. Sequences shown were inserted at the XbaI site. The Et1F sequence, when transcribed, would be complementary to the 5′end of EBER1 and represents a potential target for EBER1₂₃. The reverse (Et1R) has some complementarity to the 3′ end of EBER1. Et2 sequences are the equivalent, but include four-point changes (indicated with arrows). (B) EBER1 co-transfection (vector p661) with the luciferase reporter constructs shows no significant difference in relative luciferase activity (%RLU) expressed in either the EBV negative BL cell line BL2 or the EBV positive counterpart (BL2/B95-8). Data shown are from three independent experiments and error bars show standard deviation.