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. 2018 Apr 26;9:414. doi: 10.3389/fphar.2018.00414

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Copyright © 2018 Kirla, Groh, Poetzsch, Banote, Stadnicka-Michalak, Eggen, Schirmer and Kraemer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mCPP concentrations quantified by LC-MS/MS in the whole-body homogenates and dissected tissues of wild type and hypo-pigmented fish. Wild-type, albino and PTU-treated fish at 5 dpf (16 larvae per treatment) were either exposed to 5 μM mCPP or unexposed as controls for 8 h and mCPP was quantified in larval whole-body homogenates (A) or in dissected brain (B), trunk (C), and eyes (D). Values are reported as original data with each data point representing the information obtained from a pool of 16 larval tissues in one of the two or three independent experiments. Horizontal lines represent the mean of the experimental replicates. One-way ANOVA followed by Tukey’s multiple comparison test revealed significant difference between albino versus wild-type and PTU versus wild-type fish both for whole-body homogenates and all dissected tissues (^∗∗∗p < 0.0001).