Figure 1.

Indices of silica-induced lung pathology in diversity outbred mice. Mice were exposed transorally to 0 mg (n = 70), 5 mg (n = 90), or 10 mg (n = 120) of crystalline silica in PBS for 12 weeks before H&E stained lung sections were reviewed and scored for (A) total lung score, (B) alveolitis, and (C) peribronchitis and perivasculitis. Images of slides with (D) H&E staining of the left lung lobe from mice exposed to 0 (left), 5 (middle), and 10 mg (right) of crystalline silica in PBS. Trichrome staining of the (E) left lung lobe and (F) tracheobronchial lymph node with white arrows indicating granulomas containing lattice-like fibrous connective tissue (inset with red outline).