Figure 3.

Indices of silica-induced autoimmunity in diversity outbred mice. Mice were exposed transorally to crystalline silica in PBS (see Figure 1) before blood was collected for determination of serum (A) IgG and (B) IgM and (C) spleen weight. (D) Glomerulonephritis was determined by reviewing and scoring Periodic Acid–Schiff-stained kidney sections and (E) proteinuria was measured in the urine using Chemstrips. (F) Blood was collected prior to exposure and every 4 weeks thereafter for determination of serum anti-nuclear antibodies by immunofluorescence.