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. Author manuscript; available in PMC: 2018 May 3.
Published in final edited form as: Technology (Singap World Sci). 2016 Sep;4(3):201–215. doi: 10.1142/S2339547816500084

Figure 1.

Figure 1

Experimental schematics. (a) MSC culture medium was replaced by medium without or with IL-1β, LPS or both for 1, 6, or 24 hours. (b) Pre-activated cell culture supernatants were replaced by fresh medium for 6 or 24 hours. (c) MSC culture medium was replaced by medium without or with IL-1β for 1 hour. Pre-activated cell culture supernatants were replaced by fresh medium without or with IL-1β, TNF-α, IFN-γ, or LPS for 24 hours. (d) MSC culture medium was replaced by medium without or with IL-1β, TNF-α, IFN-γ, or LPS for 1 hour. Pre-activated cell culture supernatants were replaced by fresh medium without or with the same factor. (e) MSC transwell culture medium was replaced by medium without or with IL-1β or IFN-γ for 1 hour, and then transwells were washed and transferred to macrophage cultures with LPS for 48 hours. (f) MSC culture medium was replaced by medium without or with IL-1β or IFN-γ for 1 hour. Pre-activated cell culture supernatants were replaced by fresh medium without or with IL-1β, TNF-α, IFN-γ, or LPS for 24 hours.