Figure 6.

Response of pre-activated MSCs from multiple donors to secondary pro-inflammatory stimuli. MSCs were cultured without (black bars) or with IL-1β (grey bars) or IFN-γ (white bars) for 1 hour. PGE2 was quantified 24 hours after pre-activated cell culture supernatants were replaced by fresh medium without or with IL-1β, TNF-α, IFN-γ, or LPS. Data are the mean ± SEM for PGE2 normalized to cell number for n = 3 (Donors 2–6) or n = 9 (Donor 1) replicates.