Figure 7.

Inhibition of cPLA₂ in vivo in SE rats. A, Western blot showing MMP-2, MMP-9, ZO-1, occludin, claudin-1, and claudin-5 in brain capillaries isolated from control rats (Ctrl) and from rats that experienced pilocarpine-induced SE, that received pilocarpine but did not develop SE (Pilo), and that experienced SE and received the cPLA₂ inhibitor ATK (SE + ATK). B, Texas Red leakage in brain capillaries from control rats (n = 6), pilocarpine control rats (n = 4), SE control rats (n = 6), and ATK-treated SE rats (n = 6). High osmotic mannitol was used as positive marker for capillary leakage. Data are 0–255 AFU and are presented as mean ± SEM for n = 7 brain capillaries per time point from pooled tissue per group.