Table 3.

Western blot analysis of MMP and tight junction protein expression levels in brain capillaries exposed to glutamate (Fig. 3A)^a

Protein	Control	50 μm glutamate	100 μm glutamate
MMP-2	100 ± 11.2	91.1 ± 9.7	191.2 ± 8.4*
MMP-9	100 ± 2.0	102.4 ± 2.1	169.2 ± 3.6*
ZO-1	100 ± 6.4	92.8 ± 6.4	42.3 ± 3.3*
Occludin	100 ± 0.9	102.8 ± 1.0	86.4 ± 0.8*
Claudin-1	100 ± 2.3	100.5 ± 5.2	59.8 ± 2.5*
Claudin-5	100 ± 15.6	92.4 ± 17.1	83.9 ± 2.9*

^aData were normalized to β-actin levels; values are percentage control ± SD (n = 3).

*p < 0.05, each endpoint versus control (Dunnett's many-to-one t test).