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. 2018 Apr 26;9:566. doi: 10.3389/fpls.2018.00566

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Copyright © 2018 Cano, Sánchez-García, Albacete, González-Bayón, Justamante, Ibáñez, Acosta and Pérez-Pérez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential auxin biosynthesis and auxin signaling in mature leaves at harvest. (A) Scheme of a carnation stem cutting leaf with indication of the two regions studied. (B) Indole-3-pyruvic acid (IPyA) and (C) indole-3-acetic acid (IAA) were measured in mature leaves of carnation stem cuttings at severance time. Average ± standard deviation values are shown. (D,F) RT-qPCR of the expression of selected transcripts related to (D,E) auxin biosynthesis or (F) auxin signaling in mature leaves of carnation stem cuttings at harvesting time. Bars indicate normalized expression levels ± standard deviation relative to the distal region of the leaf in the “Master” cultivar. Letters indicate significant differences between samples (P < 0.05).