Fig. 7.

Effects of mTORC1 inhibition on intestinal barrier function in tunicamycin-challenged cells. Effect of rapamycin on tunicamycin-induced (a) transepithelial electric resistance (TEER), and (b) permeability in IEC-6 cells. IEC-6 monolayer cells incubated with tunicamycin (1 μg/mL) in the presence or absence of rapamycin (100 nmol/L) for 24 h. TEER and permeability were determined. Data are expressed as mean ± SEM, n = 3. TEER, transepithelial electrical resistance; FITC, fluorescein isothiocyanate; Rap, rapamycin. (c) Proposed mechanism for tunicamycin-induced apoptosis in intestinal epithelial cells and its feed-back regulation by mTORC1. TUN, tunicamycin