Table 2.
Comparison of three methods for determination of multiplicity of infection (MOI) of P. vivax: long fragment (422 bp) of pvmsp1 amplicon deep sequencing, short fragment (117 bp) of pvmsp1 amplicon deep sequencing, and microsatellite marker genotyping
| Amplicon deep sequencing Long fragment (422 bp) |
Amplicon deep sequencing Short fragment (117 bp) |
Microsatellite markersa | |
|---|---|---|---|
| Number of subject | 135 | 135 | 58 |
| Median MOI | 2 | 1 | 1 |
| Mean MOIa | 2.16a | 1.64b | 1.07c |
| Max MOI | 6 | 4 | 3 |
| No. polyclonal | 84 | 62 | 3 |
| % polyclonal | 62.2 | 45.9 | 5.2 |
| No. alleles | 88 | 29 | 24 |
| Heterozygosity (He) | 0.92 | 0.84 | 0.77 |
Significant differences were detected in mean MOI among the three methods as indicated by the superscripts (Tukey–Kramer HSD test, P < 0.05)
He: Expected heterozygosity corrected for sample size
aRefer to Lo et al. (2015) [39]