Fig. 3.

Absence of Fcγ receptor (FcγR)I,II,III,IV does not affect the percentage of osteoclast precursors and their osteoclastogenic potential. a Comparable percentages of CD11b^posLy6C^high and CD11b^low/negLy6C^high osteoclast precursors were present in the bone marrow of wild-type (WT) and FcγRI,II,III,IV^−/− mice at days 7 and 21 of antigen-induced arthritis (AIA) (n = 3 or 4 mice/group). b Images of tartrate-resistant acid phosphatase (TRAP) staining of osteoclasts after 5 days of in vitro differentiation. Quantification showed comparable numbers of osteoclasts obtained from WT and FcγRI,II,III,IV^−/− bone marrow cells (n = 5 mice/group). c Moreover, comparable messenger RNA expression levels of various osteoclast markers were determined in WT and FcγRI,II,III,IV^−/− osteoclasts (n = 3 mice/group). d Finally, TRAP activity in the culture supernatant of WT and FcγR I,II,III,IV^−/− macrophages and osteoclasts was comparable (n = 3 mice/group). e Representative photomicrographs of TRAP staining and quantification of the number of positive cells along the bone surface in total knee joint sections of WT and FcγRI,II,III,IV^−/− mice at days 7 and 21 after induction of AIA (n = 14 and n = 10 per time point for WT and FcγRI,II,III,IV^−/− mice, respectively). Scatterplots are shown, with horizontal and vertical lines representing mean ± SEM values. ns Not significant