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. 2018 May 2;18:514. doi: 10.1186/s12885-018-4249-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Knockdown of CBRP-1 reduced the cell proliferation and invasion ability in CAL-27 and SCC-25 cells. a The relative expression levels of CRBP-1 in TSCC cell lines were evaluated by western blotting. b Immunoblotting analysis of CRBP-1 in CAL-27 and SCC-25 cells transduced with two specific shRNA. c Growth curves of CAL-27 and SCC-25 cells with knockdown of CRBP-1. Scramble (sc): the lentiviral vector with a scrambled sequence that does not target any mRNA. d Images (upper panel) and quantification (lower panel) of invaded TSCC cells treated with melatonin for 24 h were analyzed in a transwell matrix penetration assay. Scar bar: 100 μm. Data is presented as mean ± S.D. **P < 0.01 versus the corresponding control groups