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. 2017 Dec 31;37(6):926–930. doi: 10.5851/kosfa.2017.37.6.926

Table 1. Oligonucleotides used for genotyping and RT-PCR.

Application Gene name Sequence (5′ → 3′)
Genotyping AGPAT5 Allele-specific Oligo1 ACTTCGTCAGTAACGGACGTCGAAAGCCACTGTAACATCGTAAAT
Allele-specific Oligo2 GAGTCGAGGTCATATCGTGTCGAAAGCCACTGTAACATCGTAAAC
Locus-specific Oligo GCATCTAAATAACTCTTCATAGAATCCATGAGCGGGTTCGTACCAG
TCGTCTGCCTATAGTGAGTC

RT-PCR AGPAT5 Forward TTTTCTCAGCATGGAGGGAT
Reverse GGCCTTTTTGAGCAGCAAAT
PPIA Forward CACAAACGGTTCCCAGTTTT
Reverse TGTCCACAGTCAGCAATGGT