Table 1. Oligonucleotides used for genotyping and RT-PCR.
Application | Gene name | Sequence (5′ → 3′) | |
---|---|---|---|
Genotyping | AGPAT5 | Allele-specific Oligo1 | ACTTCGTCAGTAACGGACGTCGAAAGCCACTGTAACATCGTAAAT |
Allele-specific Oligo2 | GAGTCGAGGTCATATCGTGTCGAAAGCCACTGTAACATCGTAAAC | ||
Locus-specific Oligo | GCATCTAAATAACTCTTCATAGAATCCATGAGCGGGTTCGTACCAG | ||
TCGTCTGCCTATAGTGAGTC | |||
RT-PCR | AGPAT5 | Forward | TTTTCTCAGCATGGAGGGAT |
Reverse | GGCCTTTTTGAGCAGCAAAT | ||
PPIA | Forward | CACAAACGGTTCCCAGTTTT | |
Reverse | TGTCCACAGTCAGCAATGGT |