Table I.
Effects of Triton X-114 on l-GLD activity distribution
| Enzyme | Aqueous Phasea | Detergent Phaseb | Ratio |
|---|---|---|---|
| nmol min−1 | % | ||
| l-GLD | 4.5 ± 0.28 | 19 ± 0.23 | 80.9 |
| Cyt c oxidase | 10.22 ± 0.19 | 180 ± 5.6 | 94.6 |
| NADH-Cyt c oxidoreductase | 6.4 ± 0.3 | 173.59 ± 14.39 | 96.4 |
Pure mitochondrial fractions were subjected to Triton X-114 treatment, and l-GLD activity was determined in the aqueous and detergent phases. For comparative purposes, Cyt c oxidase and NADH-Cyt c oxidoreductase activities were also determined. Activities represent means ± sd from three independent experiments. Ratio is defined as the proportion of activity at the detergent phase with respect to the total (aqueous plus detergent phases).
a
Total protein, 0.7 mg.
b
Total protein, 0.98 mg.