Table II.
Distribution of l-GLD activity in submitochondrial fractions
| Enzyme | MMa | IMb | OMc | ISd |
|---|---|---|---|---|
| nmol min−1 | ||||
| l-GLD | 3.74 ± 0.3 | 39.9 ± 1.59 | 3.7 ± 1 | NDe |
| Cyt c oxidase | 192.4 ± 30 | 1305 ± 28 | 84.3 ± 1.7 | ND |
| NADH-Cyt c oxidoreductase | 12.1 ± 0.3 | 17.5 ± 0.4 | 59.4 ± 1.1 | 4.02 ± 0.02 |
Submitochondrial fractions were isolated from pure mitochondria. l-GLD, Cyt c oxidase (as a marker of inner membrane), and NADH-Cyt c oxidoreductase (as a marker of outer membrane) activities were determined. Data represent means ± sd from three independent experiments.
a
MM, Mitochondrial matrix (1.4 mg total protein).
b
IM, Inner membrane (1.75 mg total protein).
c
OM, Outer membrane (0.36 mg total protein).
d
IS, Intermembrane space (0.9 mg total protein).
e
ND, Not detected.