Table 2.
ROS evaluation of ethidium and DCF fluorescence in frozen-thawed boar sperm of control and astaxanthin (AXT).
| Sperm treatment | Viable sperm with high O2− (%) | MFI of ethidium/total sperm | MFI of ethidium/viable sperm | Viable sperm with high H2O2− (%) | MFI of DCF/total sperm | MFI of DCF/viable sperm |
|---|---|---|---|---|---|---|
| Control | 31.5 ± 1.8a | 116.8 ± 20.4 | 135.0 ± 24.0 | 4.0 ± 0.8 | 72.2 ± 0.9ab | 87.7 ± 2.1ab |
| AXT 10 μM | 44.4 ± 1.0c | 106.9 ± 22.8 | 114.0 ± 23.0 | 4.8 ± 0.9 | 83.0 ± 8.2b | 95.7 ± 7.2b |
| AXT 50 μM | 43.7 ± 1.6bc | 118.0 ± 6.6 | 126.6 ± 13.8 | 4.0 ± 0.8 | 73.8 ± 6.8ab | 87.8 ± 8.0ab |
| AXT 100 μM | 41.1 ± 0.9bc | 116.7 ± 10.5 | 121.2 ± 9.9 | 2.7 ± 0.9 | 65.6 ± 4.3a | 76.0 ± 4.5a |
| AXT 500 μM | 40.8 ± 0.1b | 124.3 ± 16.1 | 130.7 ± 16.4 | 4.3 ± 0.3 | 78.0 ± 2.3ab | 95.1 ± 2.4b |
| p value | p < 0.001 | p > 0.05 | p > 0.05 | p > 0.05 | p < 0.05 | p < 0.01 |
a, b, and c indicate difference P < 0.001, P < 0.05, and P < 0.01, respectively.