Figure 2.
T regulatory cell (Treg) depletion in peripheral blood and uterine tissue is equivalent between 1, 2, and 3 days of injection. Whole-blood Tregs nadir occurs earlier compared to uterine tissue nadir. (A) Comparison of whole-blood FoxP3 percentages after 1, 2, or 3 days of 1 μg intraperitoneal (IP) diphtheria toxin (DT) injection at days 0 to 3 and 10 days post day 1 injection. (B) Comparison of uterine tissue percentages of FoxP3 after 1, 2, or 3 days of IP DT injection at days 0 to 3 and 10 days postinjection. (C) Comparison of whole-blood and uterine percentages after 2 days of IP DT injection at days 0 to 3, 10, 21, and 28 days postinjection. Significant differences (P < .05) were noted between day 0 and day 3 in whole-blood percentages and between day 0 and day 10 and day 21 uterine tissue percentages. (D-H) Flow cytometry analysis of FoxP3 antibody staining. (D) Day 0—whole blood, (E) whole blood postinjection day 3, (F) day 0—uterine tissue, (G) uterine tissue postinjection day 10, and (H) uterine tissue post injection day 21. Student t test was used for analysis. Experiments were performed in triplicate.