Figure 3.
Conditional knockdown of T regulatory cells (Tregs) followed by immediate mating timed to whole-blood Treg nadir leads to a subtle implantation defect, where fewer embryos are able to implant (as evidenced by smaller litter sizes) but showed similar pregnancy rates (breeding efficiency) overall. Tregs knockdown with delayed mating timed to uterine Treg nadir results in a more pronounced reproductive phenotype, where the overall breeding efficiency was significantly decreased. Breeding efficiency (A) and litter size (B) comparison after conditional Tregs knockdown and immediate mating timed to blood Treg nadir. Breeding efficiency (C) and litter sizes (D) after conditional Tregs knockdown and delayed mating timed to uterine Treg nadir. Analysis of variance with Tukey test for multiple comparisons was used for analysis. *P < .05, **P < .01.