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Allogeneic mating timed to blood T regulatory cells (Tregs) knockdown significantly decreased breeding efficiency. (A) Litter size comparison after conditional knockdown and immediate mating with BALB/c wild-type (WT) male. (B) Breeding efficiency (pregnancy rates) after conditional knockdown and immediate mating with BALB/c WT male. Analysis of variance with Tukey test for multiple comparisons was used for analysis. *P < .05.
