Figure 7.

ASC/caspase (CASP)-1 complex formation in the chorioamniotic membranes. In situ proximity ligation assays for ASC and CASP-1 in the chorioamniotic membranes from women in spontaneous preterm labor with (PTL-ACA) or without (PTL) acute histologic chorioamnionitis. (A and B) Representative images of ASC/CASP-1 complexes (red signal) in PTL and PTL-ACA groups. (D and E) Representative images of isotype controls in PTL and PTL-ACA groups. The blue signal is DAPI (nuclei). 400× magnifications. C, Semi-quantification of the number of ASC/CASP-1 complexes in the chorioamniotic membranes (PTL and PTL-ACA, n = 7 each). ASC/CASP-1 complexes were calculated by dividing the number of signals over the area of the tissue, which was expressed as pixels. Mann-Whitney U tests.