Skip to main content
. 2015 Sep 3;23(3):352–364. doi: 10.1177/1933719115602770

Figure 3.

Figure 3.

Expression of endoplasmic reticulum (ER) stress-associated genes in mouse granulosa cells treated with follicle stimulating hormone (FSH). A-E, Mouse granulosa cells in culture were treated with indicated doses of FSH for 24 or 48 hours. Total RNA was extracted and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was performed for ER stress associated genes. F, Mouse granulosa cells in culture were treated with 100 mIU/mL FSH for 48 hours. Activating transcription factor 4 (ATF4) Western blot band intensity is normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Data are the mean ± standard error of the mean (SEM). Gene names are as in Figure 2. Column bars with asterisk are significantly different from control (P < .05). One-way analysis of variance (ANOVA) was used to determine statistical significance of quantitative polymerase chain reaction (qPCR) experiments. Unpaired t test was used to determine statistical significance of the difference in ATF4 Western blot band intensity.