Figure 6.

The effect of endoplasmic reticulum (ER) stress induction with thapsigargin on granulosa cell follicle stimulating hormone (FSH) response. Mouse granulosa cells in culture were treated with thapsigargin and/or FSH for 60 hours. A, Total RNA was extracted and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was performed for ER stress associated genes. Data are the mean ± standard error of the mean (SEM). B, Estradiol production was assessed by enzyme-linked immunosorbent assay (ELISA). Data are the mean ± standard error of the mean (SEM). C, Aromatase expression was assessed by PCR. Actin was used as a control. Gene names are as in Figures 2, 3, and 5. Asterisks indicate statistical significance (P < .05). Unpaired t test was used to determine statistical significance of quantitative polymerase chain reaction (qPCR) and estradiol production experiments. Tp indicates thapsigargin.