Table 4.
Tissue Thickness Versus TEER With Hormone Treatment.
| Hormone Treatment | VEC-FT | VEC-PT | ||||
|---|---|---|---|---|---|---|
| Reproductive Hormone | Concentration (nm) | Mean Tissue Thickness (μm) ± SD | Mean TEER (Ω × cm2) ± SD | Mean Tissue Thickness (μm) ± SD | Mean TEER (Ω × cm2) ± SD | |
| Untreated Control | 0 | 107 ± 26.8 | 109 ± 13.7 | 197 ± 12.8 | 91 ± 9.8 | |
| Estradiol | 100 | 188 ± 8.8** | 134 ± 7.8** | 276 ± 28.7* | 120 ± 11.7** | |
| Progesterone | 100 | 71 ± 36.5 | 93 ± 7.8** | 112 ± 24.5** | 61 ± 7.3** | |
| Estradiol+ progesterone | 10:10 | 103 ± 24.0 | 112 ± 11.3 | 167 ± 57.6 | 92 ± 14.1 | |
| * = P < .01 | ||||||
| ** = P < .005 | ||||||
Abbreviations: VEC-PT, partial thickness vaginal/ectocervical tissue; VEC-FT, full thickness vaginal/ectocervical tissue; SD, standard deviation; TEER, transepithelial electrical resistance.
a Effect of endocrine hormones on tissue thickness and TEER in 3 separate experiments (3 lots). Vaginal-ectocervical tissues EpiVaginal tissues full (FT) and partial (PT) thickness were cultured for 11 days in medium supplemented with hormones. At the end of the culture period of each lot, tissue thickness was measured at 10 different locations and an average thickness for the 3 lots was calculated. Estradiol treatment resulted in increased tissue thickness and TEER in the VEC-FT and VEC-PT tissue models.