Table 1.
Primer Sequences for L-Type Ca2+ Channels, Sarcoplasmic Ca2+ ATPase, Na+/Ca2+ Exchanger, Ryanodine Receptors, Protein Kinase C Isoforms, and Transient Receptor Potential Channels.
| Gene Name | Forward (5′→3′) | Reverse (5′→3′) |
|---|---|---|
| Cacna1c | GATTCGATGTGAAGGCACTGAGAGC | ATGGCCTTGATGATGGAGTTCAGGA |
| Cacna1d | AATCAGGAGGCTTTGATGTCAAAGCC | AGGGGGACCATGGCTTTTATAATGGAG |
| Cacna1s | CCATCATCTTGCTCACCATCTTCGC | GAGAAGACGATGAGGAAGAAGTACTCCAG |
| Cacna1f | TGAGCTACCTTGGGTGTACTTTGTGAG | GCTTCTCCCGAAGCTTCTGAAAGTC |
| Ryr1 | CGAGGACGAGGTCCAGTTTTTGCGGACG | ACACAACTTGAGCTGCTCCTTGAGCA |
| Ryr2 | GATGTCAAATCAGCACGAATGGGATCC | TCGTGCTGCGATCTGGATAAGTTCA |
| Ryr3 | AATGCTTGCCAACACCGTTGAAAATGG | CTGAAGGAGTGCCGCAGGAGAATTGCA |
| Pkc α | GATTTACCTGAAGGCAGAGGTCACAGAT | GGGTCAGGAATAAGTTTCAGCTTCACGTA |
| Pkc δ | GCCTTTCTGTGCCGTGAAGATGA | ATAGATGTGGGCGTCGAATGTTGACT |
| Pkc ∊ | GCGGAAACACCCTTATCTAACCCAAC | GGAACATGAGGTCTCCACCGTTTACATA |
| Trpc1 | ATATACTGCAGCTGCTTTTGGACTACGG | GTGATGATCGTTTTGGCCGATGGTTAAG |
| Trpc2 | ATAGCCATGGAGAACCAGATTCCAGTC | ACTTTCCTCCTCTCCTTGTT |
| Trpc3 | GGCCAAAGTAAACCCTGCTTTTACCAC | GACAACAGAAGTCACTTCAGAGAGTCCAAATA |
| Trpc4 | GAGTGGATGATATTACCGTGGGTCCT | CACCAGTCGTGGATGTAATCCTGAAGT |
| Trpc5 | CCAAGCTGAAGGTGGCAATCAAATATCAC | AGTGTTTTCTTCGCCATCCAGGGAA |
| Trpc6 | TTTAGAACTCAGCAATGAGCTGGCAGT | TCCAGGAGACCCACAACAAAATCCTT |
| Trpc7 | GCATCAAACTTGCCATTAAGTACGAAGTCAA | GATAGACTGTTGCCGTAAGCCTGAGAG |
| Atp2a1 | ATGACCATGGCCTTGTCTGTGTTG | AGATGGAACCGAGAAGCCAGATGTT |
| Atp2a2 | TTGCTGGAACTTGTGATCGAGCAGT | GGCTGTAATCGTTTCTTCACCTTCTTCG |
| Atp2a3 | TGTACGTAGGCCTGGCTACAGTG | TGGATTGTCTTCAGAGCACTTCAGGAAG |
| Slc8A1 | CATTCTAGGCGAACACACCAAGCTG | GGCCAGGTTCGTCTTCTTAATGAGTTTG |
| Slc8A2 | GTTTTTAGAGGCAGTTACAGTGAGCGC | AAGTGCATCACGTAGTCAAAGCAGGAT |
| Slc8A3 | GGGAGCTGGAGTTTAAGAATGATGAAACG | CAAGGGCAATGAAGAAATTCTCTTGCCT |
Abbreviations: Atp, ATPases; Pkc, protein kinase C; Ryr, ryanodine receptors; Slc, Na+/Ca2+ exchangers; Trpc: transient receptor potential channels.