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Import of BAT2 protein into isolated chloroplasts. 35S-Labeled proteins derived from an in vitro transcription-translation programmed by the BAT2 cDNA (lane Tr) were incubated with intact chlorplasts (lane C), treated with thermolysin (lane C+), and fractionated into stromal (lane S) and membrane (lane M) fractions. preBAT2, Preprotein; BAT2, mature protein. On the right are the apparent molecular masses in kD.
