Figure 5.

Northern hybridization of B. napus BAT2 expression. Total RNA was extracted from root and stem and poly(A⁺) RNA from leaf, flower, and immature embryos isolated at 28 d after pollination. The RNAs were resolved on formamide agarose gels, blotted to a nylon membrane, and hybridized with a 632-bp probe corresponding to the truncated BAT2 coding sequence cDNA insert and subsequently with an rRNA probe. The filters were washed to a stringency of 0.125× SSC and 0.1% SDS at 60°C and autoradiographed.