Figure 3.

Flow cytometric analysis of IFN-γ expression in freshly isolated FTDCs and dNK cells from first-trimester decidua. Single-cell suspensions prepared from enzyme-digested fresh decidua were stained with antihuman CD3-V450, CD45-AF700, CD56-FITC, vimentin preeclampsia (PE), and IFN-γ-APC antibodies. A, The IFN-γ expression in dNK cells and FTDCs in steady state. B, The IFN-γ expression in dNK cells treated with IL-12 + IL-18. The results are reported as mean ± standard error of the mean (SEM). n = 3; *P < .05 and ** P < .01. dNK indicates decidual natural killer; FTDC, first-trimester decidual cell; IFN-γ, interferon γ; IL, interleukin.