Table I. Amino acid content in leaves following darkening for 6 d of an individual leaf (IDL) and an entire plant (DP).
Results are means of three replicates ± se and are expressed in μmol g−1 fresh weight. Dark treatments were compared with light samples by Student’s t test (*, significant at P < 0.05; ns, not significant). n/a, Not applicable.
| Amino Acid | Light | DP | IDL |
|---|---|---|---|
| Ammonium | 0.80 ± 0.42 | 0.18 ± 0.05 ns | 0.54 ± 0.60 ns |
| Ala | 0.64 ± 0.11 | 1.03 ± 0.17* | 0.26 ± 0.04* |
| Arg | 0.03 ± 0.06 | 2.44 ± 0.06* | 0.33 ± 0.10* |
| Asn | 0.34 ± 0.04 | 14.44 ± 1.13* | 0.99 ± 0.62 ns |
| Asp | 1.85 ± 0.20 | 0.85 ± 0.25* | 0.22 ± 0.04* |
| Cys | n/a | n/a | n/a |
| γ-Aminobutyric acid | 0.00 ± 0.00 | 0.36 ± 0.03* | 0.17 ± 0.05* |
| Gln | 2.11 ± 0.19 | 2.93 ± 0.45 ns | 0.67 ± 0.27* |
| Glu | 2.15 ± 0.07 | 4.40 ± 0.34* | 1.86 ± 0.05* |
| Gly | 0.27 ± 0.07 | 0.00 ± 0.00* | 0.03 ± 0.03* |
| His | 0.00 ± 0.00 | 0.85 ± 0.03* | 0.05 ± 0.08 ns |
| Ile | 0.00 ± 0.00 | 0.99 ± 0.17* | 0.05 ± 0.05 ns |
| Leu | 0.11 ± 0.00 | 0.54 ± 0.07* | 0.22 ± 0.02* |
| Lys | 0.05 ± 0.09 | 0.47 ± 0.05* | 0.26 ± 0.03 ns |
| Met | n/a | n/a | n/a |
| Phe | 0.00 ± 0.00 | 2.06 ± 0.08* | 0.12 ± 0.02* |
| Pro | 0.79 ± 0.38 | 0.06 ± 0.02 ns | 0.07 ± 0.01 ns |
| Ser | 1.30 ± 0.22 | 2.04 ± 0.40 ns | 0.88 ± 0.15 ns |
| Thr | 0.60 ± 0.14 | 1.16 ± 0.38 ns | 0.37 ± 0.08 ns |
| Trp | 0.00 ± 0.00 | 0.88 ± 0.02* | 0.15 ± 0.04* |
| Tyr | 0.01 ± 0.02 | 0.40 ± 0.04* | 0.08 ± 0.01* |
| Val | 0.10 ± 0.00 | 2.05 ± 0.28* | 0.19 ± 0.02* |
| Total | 11.17 | 38.14 | 7.51 |