Figure 2.

Expression levels of ACCase-encoding genes in Arabidopsis T87 cells were not significantly affected 4 d after exposure to 10 mm Tween 80 (A) or after 4 d of exposure to Tween 80 followed by 2 d of incubation in Tween 80-free medium (B). RT-qPCR values are presented as percentages of untreated controls normalized as described in “Materials and Methods.” All data are means ± sd (n = 3), and no values were found to differ significantly (P < 0.05) from controls using mean crossing point deviation analysis computed by the relative expression (REST) software algorithm (Pfaffl et al., 2002).