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. 2018 Mar 26;177(1):255–270. doi: 10.1104/pp.17.01759

Figure 1.

Figure 1.

The rmd-1 mutant has abnormal pollen germination and pollen tube growth in vitro. A and B, Micrographs of pollen tubes were taken 10 min after germination in vitro. Pollen grains derived from wild-type (WT; A) and rmd-1 (B) plants were germinated in the standard liquid germination medium. Bars = 100 μm. C, In vitro germination percentages of wild-type and rmd-1 pollen grains (n > 200 pollen grains for each genotype). D and E, Wild-type (D) and rmd-1 (E) pollen tubes were visualized by a microscope 10 min after germination in vitro. Bars = 10 μm. F and G, The average pollen tube length (F) and width (G) of wild-type and rmd-1 plants (only germinated pollen grains were taken into account) were analyzed 10 min after germination (n > 150 for each genotype). H, Graph of pollen tube growth rates of the wild type and the rmd-1 mutant between 3 and 10 min after germination in vitro (each genotype, n > 35). For C and F to H, error bars represent sd of at least three independent experiments. Student’s t test: **, P < 0.01.